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hiscribe t7 arca mrna kit  (New England Biolabs)


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    New England Biolabs hiscribe t7 arca mrna kit
    Safety and induced antibody isotypes of the <t>mRNA</t> vaccine in lactating dairy cows. Six cows were immunized intramuscularly with 500 μg of mRNA–LNP twice, 3 weeks apart. (A) Daily milk yield in vaccinated cows and unvaccinated controls, recorded from 3 d before the first immunization to 14 d after the booster. (B and C) Antibody isotypes in serum and milk measured by ELISA:IgG (B) and IgA (C) using bovine IgG- and IgA-specific detection antibodies. OD, optical density.
    Hiscribe T7 Arca Mrna Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 338 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiscribe t7 arca mrna kit/product/New England Biolabs
    Average 99 stars, based on 338 article reviews
    hiscribe t7 arca mrna kit - by Bioz Stars, 2026-02
    99/100 stars

    Images

    1) Product Images from "Protective Efficacy of a Hemagglutinin-Based mRNA Vaccine Against H5N1 Influenza Virus Challenge in Lactating Dairy Cows"

    Article Title: Protective Efficacy of a Hemagglutinin-Based mRNA Vaccine Against H5N1 Influenza Virus Challenge in Lactating Dairy Cows

    Journal: Research

    doi: 10.34133/research.1104

    Safety and induced antibody isotypes of the mRNA vaccine in lactating dairy cows. Six cows were immunized intramuscularly with 500 μg of mRNA–LNP twice, 3 weeks apart. (A) Daily milk yield in vaccinated cows and unvaccinated controls, recorded from 3 d before the first immunization to 14 d after the booster. (B and C) Antibody isotypes in serum and milk measured by ELISA:IgG (B) and IgA (C) using bovine IgG- and IgA-specific detection antibodies. OD, optical density.
    Figure Legend Snippet: Safety and induced antibody isotypes of the mRNA vaccine in lactating dairy cows. Six cows were immunized intramuscularly with 500 μg of mRNA–LNP twice, 3 weeks apart. (A) Daily milk yield in vaccinated cows and unvaccinated controls, recorded from 3 d before the first immunization to 14 d after the booster. (B and C) Antibody isotypes in serum and milk measured by ELISA:IgG (B) and IgA (C) using bovine IgG- and IgA-specific detection antibodies. OD, optical density.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Protective efficacy of the H5 mRNA–LNP vaccine in lactating dairy cows 5 weeks p.v. Vaccinated and unvaccinated dairy cows were challenged with a dairy cow H5N1 virus via intranasal and intramammary routes. A dose of 2 × 10 6 EID 50 of virus was administered intranasally (1 ml per nostril), and different doses were administered to individual mammary quarters via the teat: 1 ml of PBS into the left front (LF) quarter, 1 ml (10 2 EID 50 ) into the left rear (LR) quarter, 1 ml (10 4 EID 50 ) into the right front (RL) quarter, and 1 ml (10 6 EID 50 ) into the right rear (RR) quarter. Nasal swabs, saliva, milk, and tissues were harvested at the indicated times postchallenge (p.c.) and titrated in embryonated chicken eggs. All panels show viral titers in (A) nasal swabs, (B) saliva, (C) milk, and tissues (D and E). Each bar denotes an individual cow. The lower detection threshold is shown as a horizontal dashed line.
    Figure Legend Snippet: Protective efficacy of the H5 mRNA–LNP vaccine in lactating dairy cows 5 weeks p.v. Vaccinated and unvaccinated dairy cows were challenged with a dairy cow H5N1 virus via intranasal and intramammary routes. A dose of 2 × 10 6 EID 50 of virus was administered intranasally (1 ml per nostril), and different doses were administered to individual mammary quarters via the teat: 1 ml of PBS into the left front (LF) quarter, 1 ml (10 2 EID 50 ) into the left rear (LR) quarter, 1 ml (10 4 EID 50 ) into the right front (RL) quarter, and 1 ml (10 6 EID 50 ) into the right rear (RR) quarter. Nasal swabs, saliva, milk, and tissues were harvested at the indicated times postchallenge (p.c.) and titrated in embryonated chicken eggs. All panels show viral titers in (A) nasal swabs, (B) saliva, (C) milk, and tissues (D and E). Each bar denotes an individual cow. The lower detection threshold is shown as a horizontal dashed line.

    Techniques Used: Virus

    Protective efficacy of the H5 mRNA–LNP vaccine in lactating dairy cows 19 weeks p.v. Vaccinated and unvaccinated dairy cows were challenged with a dairy cow H5N1 virus via intranasal and intramammary routes. A dose of 2 × 10 6 EID 50 of virus was administered intranasally (1 ml per nostril), and different doses were administered to individual mammary via the teat: 1 ml of PBS into the left front quarter, 1 ml (10 2 EID 50 ) into the left rear quarter, 1 ml (10 4 EID 50 ) into the right front quarter, and 1 ml (10 6 EID 50 ) into the right rear quarter. Nasal swabs, saliva, milk, and tissues were harvested at the indicated times postchallenge and titrated in embryonated chicken eggs. All panels show levels of virus in (A) nasal swabs, (B) saliva, (C) milk, and tissues (D and E). Each bar denotes an individual cow. The lower detection threshold is shown as a horizontal dashed line.
    Figure Legend Snippet: Protective efficacy of the H5 mRNA–LNP vaccine in lactating dairy cows 19 weeks p.v. Vaccinated and unvaccinated dairy cows were challenged with a dairy cow H5N1 virus via intranasal and intramammary routes. A dose of 2 × 10 6 EID 50 of virus was administered intranasally (1 ml per nostril), and different doses were administered to individual mammary via the teat: 1 ml of PBS into the left front quarter, 1 ml (10 2 EID 50 ) into the left rear quarter, 1 ml (10 4 EID 50 ) into the right front quarter, and 1 ml (10 6 EID 50 ) into the right rear quarter. Nasal swabs, saliva, milk, and tissues were harvested at the indicated times postchallenge and titrated in embryonated chicken eggs. All panels show levels of virus in (A) nasal swabs, (B) saliva, (C) milk, and tissues (D and E). Each bar denotes an individual cow. The lower detection threshold is shown as a horizontal dashed line.

    Techniques Used: Virus



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    Image Search Results


    Safety and induced antibody isotypes of the mRNA vaccine in lactating dairy cows. Six cows were immunized intramuscularly with 500 μg of mRNA–LNP twice, 3 weeks apart. (A) Daily milk yield in vaccinated cows and unvaccinated controls, recorded from 3 d before the first immunization to 14 d after the booster. (B and C) Antibody isotypes in serum and milk measured by ELISA:IgG (B) and IgA (C) using bovine IgG- and IgA-specific detection antibodies. OD, optical density.

    Journal: Research

    Article Title: Protective Efficacy of a Hemagglutinin-Based mRNA Vaccine Against H5N1 Influenza Virus Challenge in Lactating Dairy Cows

    doi: 10.34133/research.1104

    Figure Lengend Snippet: Safety and induced antibody isotypes of the mRNA vaccine in lactating dairy cows. Six cows were immunized intramuscularly with 500 μg of mRNA–LNP twice, 3 weeks apart. (A) Daily milk yield in vaccinated cows and unvaccinated controls, recorded from 3 d before the first immunization to 14 d after the booster. (B and C) Antibody isotypes in serum and milk measured by ELISA:IgG (B) and IgA (C) using bovine IgG- and IgA-specific detection antibodies. OD, optical density.

    Article Snippet: Transcription was performed in vitro using the HiScribe T7 ARCA mRNA Kit (with tailing) (New England Biolabs, USA) according to the manufacturer’s protocol, with the linearized plasmid as the template.

    Techniques: Enzyme-linked Immunosorbent Assay

    Protective efficacy of the H5 mRNA–LNP vaccine in lactating dairy cows 5 weeks p.v. Vaccinated and unvaccinated dairy cows were challenged with a dairy cow H5N1 virus via intranasal and intramammary routes. A dose of 2 × 10 6 EID 50 of virus was administered intranasally (1 ml per nostril), and different doses were administered to individual mammary quarters via the teat: 1 ml of PBS into the left front (LF) quarter, 1 ml (10 2 EID 50 ) into the left rear (LR) quarter, 1 ml (10 4 EID 50 ) into the right front (RL) quarter, and 1 ml (10 6 EID 50 ) into the right rear (RR) quarter. Nasal swabs, saliva, milk, and tissues were harvested at the indicated times postchallenge (p.c.) and titrated in embryonated chicken eggs. All panels show viral titers in (A) nasal swabs, (B) saliva, (C) milk, and tissues (D and E). Each bar denotes an individual cow. The lower detection threshold is shown as a horizontal dashed line.

    Journal: Research

    Article Title: Protective Efficacy of a Hemagglutinin-Based mRNA Vaccine Against H5N1 Influenza Virus Challenge in Lactating Dairy Cows

    doi: 10.34133/research.1104

    Figure Lengend Snippet: Protective efficacy of the H5 mRNA–LNP vaccine in lactating dairy cows 5 weeks p.v. Vaccinated and unvaccinated dairy cows were challenged with a dairy cow H5N1 virus via intranasal and intramammary routes. A dose of 2 × 10 6 EID 50 of virus was administered intranasally (1 ml per nostril), and different doses were administered to individual mammary quarters via the teat: 1 ml of PBS into the left front (LF) quarter, 1 ml (10 2 EID 50 ) into the left rear (LR) quarter, 1 ml (10 4 EID 50 ) into the right front (RL) quarter, and 1 ml (10 6 EID 50 ) into the right rear (RR) quarter. Nasal swabs, saliva, milk, and tissues were harvested at the indicated times postchallenge (p.c.) and titrated in embryonated chicken eggs. All panels show viral titers in (A) nasal swabs, (B) saliva, (C) milk, and tissues (D and E). Each bar denotes an individual cow. The lower detection threshold is shown as a horizontal dashed line.

    Article Snippet: Transcription was performed in vitro using the HiScribe T7 ARCA mRNA Kit (with tailing) (New England Biolabs, USA) according to the manufacturer’s protocol, with the linearized plasmid as the template.

    Techniques: Virus

    Protective efficacy of the H5 mRNA–LNP vaccine in lactating dairy cows 19 weeks p.v. Vaccinated and unvaccinated dairy cows were challenged with a dairy cow H5N1 virus via intranasal and intramammary routes. A dose of 2 × 10 6 EID 50 of virus was administered intranasally (1 ml per nostril), and different doses were administered to individual mammary via the teat: 1 ml of PBS into the left front quarter, 1 ml (10 2 EID 50 ) into the left rear quarter, 1 ml (10 4 EID 50 ) into the right front quarter, and 1 ml (10 6 EID 50 ) into the right rear quarter. Nasal swabs, saliva, milk, and tissues were harvested at the indicated times postchallenge and titrated in embryonated chicken eggs. All panels show levels of virus in (A) nasal swabs, (B) saliva, (C) milk, and tissues (D and E). Each bar denotes an individual cow. The lower detection threshold is shown as a horizontal dashed line.

    Journal: Research

    Article Title: Protective Efficacy of a Hemagglutinin-Based mRNA Vaccine Against H5N1 Influenza Virus Challenge in Lactating Dairy Cows

    doi: 10.34133/research.1104

    Figure Lengend Snippet: Protective efficacy of the H5 mRNA–LNP vaccine in lactating dairy cows 19 weeks p.v. Vaccinated and unvaccinated dairy cows were challenged with a dairy cow H5N1 virus via intranasal and intramammary routes. A dose of 2 × 10 6 EID 50 of virus was administered intranasally (1 ml per nostril), and different doses were administered to individual mammary via the teat: 1 ml of PBS into the left front quarter, 1 ml (10 2 EID 50 ) into the left rear quarter, 1 ml (10 4 EID 50 ) into the right front quarter, and 1 ml (10 6 EID 50 ) into the right rear quarter. Nasal swabs, saliva, milk, and tissues were harvested at the indicated times postchallenge and titrated in embryonated chicken eggs. All panels show levels of virus in (A) nasal swabs, (B) saliva, (C) milk, and tissues (D and E). Each bar denotes an individual cow. The lower detection threshold is shown as a horizontal dashed line.

    Article Snippet: Transcription was performed in vitro using the HiScribe T7 ARCA mRNA Kit (with tailing) (New England Biolabs, USA) according to the manufacturer’s protocol, with the linearized plasmid as the template.

    Techniques: Virus